Plasmid
His3::EX-R
Part:BBa_K3271010:Design
Designed by: Setti Belhouari Group: iGEM19_uOttawa (2019-10-13)
His3::EX-RFP-SP pSB1K3
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 2291
Illegal suffix found in sequence at 3382 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2291
Illegal SpeI site found at 3383
Illegal PstI site found at 3397
Illegal NotI site found at 2297
Illegal NotI site found at 3390 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2291
Illegal XhoI site found at 1026
Illegal XhoI site found at 2052 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2291
Illegal suffix found in sequence at 3383 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2291
Illegal XbaI site found at 2306
Illegal SpeI site found at 3383
Illegal PstI site found at 3397
Illegal AgeI site found at 3093
Illegal AgeI site found at 3205 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Please note the upstream and downstream His3 locus homologies are found outside the prefix and suffix, not within the multiple cloning site. This contribution consists of an updated pSB1K3 BioBrick backbone that makes the BioBrick plasmids compatible with cloning in both E. coli and S. cerevisiae.
Source
The His3 upstream and downstream homologies are comprised of 100 bp each from the promoter region and the terminator region of the His3 locus of S. cerevisiae, respectively. The RFP cassette is identical in sequence to BBa_J04450.